Complement, Total, Serum
Method Description Describes how the test is performed and provides a method-specific reference
An automated method is performed using liposomes as the target for the serum complement system. The dinitrophenyl (DNP)-labeled liposomes are sensitized with antibody to DNP. Serum complement causes lysis and release of entrapped glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase reacts with glucose-6-phosphate and nicotinamide adenine dinucleotide (NAD). NAD is reduced to reduced nicotinamide adenine dinucleotide (NADH) and the conversion is measured at 340 nm. The assay correlates with the CH50 assay based on sheep RBC lysis, has lower variability, and is simpler to perform.(Package insert: Wako Autokit CH50. Wako Pure Chemical Industries, Ltd., 08/2007; Yamamoto S, Kubotsu K, Kida M, et al: Automated homogeneous liposome-based assay system for total complement activity. Clin Chem 1995;41:586-590)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 3 p.m.