Purine and Pyrimidine Panel, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Diluted, filtered urine is added to an internal standard mixture and analyzed for uracil, uric acid, xanthine, and hypoxanthine by liquid chromatography tandem mass spectrometry (LC-MS/MS). LC-MS/MS is performed using a mobile phase composed of 50-mM ammonium formate, pH=5, and 1:1 mixture of 50-mM ammonium formate, pH=5:methanol, and run using a gradient. An Xterra C18 column (2.1 x 150 mm) is used to separate xanthine and hypoxanthine from the bulk of the specimen matrix. The MS/MS is operated in the selected reaction monitoring (SRM) scanning mode. The ratios of the extracted peak areas of xanthine and hypoxanthine to an internal standard are used to calculate the concentration of xanthine and hypoxanthine present.(Ito T, Van Kuilenburg AP, Bootsma AH, et al: Rapid screening of high-risk patients for disorders of purine and pyrimidine metabolism using HPLC-electrospray tandem mass spectrometry of liquid urine or urine-soaked filter paper strips. Clin Chem 2000;46:445-452)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday; 8 a.m.