Test ID: PGSN    
Progesterone, Serum

Testing is performed on a Roche Cobas. The Roche Progesterone II assay is a competitive assay using electrochemiluminescence detection. Patient specimen, biotinylated monoclonal progesterone-specific antibody, and a progesterone derivative labeled with ruthenium complex are incubated with Danazol to release progesterone. Progesterone from the sample competes with the labeled progesterone derivative for the antibody binding site. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The amount of the labeled progesterone derivative bound to the solid phase is inversely proportional to the progesterone content of the sample. This reaction mixture is aspirated into measuring cell where the bound microparticles are captured onto the electrode surface and unbound substances are removed. Voltage is applied to the electrode inducing a chemiluminescent emission, which is then measured against a calibration curve to determine the amount of progesterone in the patient specimen.(Package insert: Roche Cobas. Roche Diagnostics, Indianapolis, IN 09/2007)

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