Lamellar Body Count Reflex, Amniotic Fluid
Method Description Describes how the test is performed and provides a method-specific reference
The Sysmex Automated Hematology Analyzer XE-5000 measures platelet count by the impedance method to quantify lamellar body counts. (Automated Hematology Analyzer XE-5000 Instructions for Use [North American Edition], Code No. 461-2638-6, Date of Last Revision: December 2011)
One-dimensional thin-layer chromatography (TLC) technique is used for separating the phospholipids in amniotic fluid. The first step in the process involves extraction of the lipids present in the amniotic fluid using chloroform/methanol mixture. The extracted specimen is streaked onto a TLC plate and placed in tank 1 for prechromatography, a step that removes a component that otherwise comigrates with phosphatidylglycerol (PG). Lipids are then separated using tank 2. The separated lipids are made visible with dilute cupric acetate and charring. Lecithin to sphingomyelin (L to S) is determined using a scanning densitometer. The relative intensities of L and S are expressed as a ratio, and PG is reported from a visual observation of the plate and reported as present, absent, or trace.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
LBC: Monday through Sunday; Continuously
Monday through Friday; 2 p.m.
Saturday and Sunday; 11 a.m.
Weekdays: Sample delivery time is 7:00 a.m. to 4:30 p.m.
Set-up time up to 2:00 p.m.
Saturday: Sample delivery time is 7:00 a.m. to 3:00 p.m.
Set-up time is up to 11:00 a.m.
Sunday: Sample delivery time is 8:00 a.m.-1:00 p.m.
Set-up time is up to 11:00 a.m. (On call only)