|Values are valid only on day of printing.|
Creatine Kinase, Total:
Creatine kinase (CK) is determined by a coupled enzyme reaction where the rate of NADPH formation is measured photometrically and is directly proportional to the CK activity.(Package insert: Roche CK reagent. Indianapolis, IN, July 1999)
Creatine Kinase Isoenzymes:
Electrophoresis is the migration of charge particles in an electrical field. The rate of migration is dependent on such factors as: 1) net electrical charge of the molecule, 2) size and shape of the molecule, 3) strength of the electrical field, 4) properties of the supporting medium, 5) temperature of the operation.
In this procedure, serum CK is separated into the 3 main isoenzymes of CK (MM, MB, and BB) by electrophoretic separation on agarose gel. After electrophoresis, the separated CK isoenzymes are visualized using a chromogenic substrate. The amount of resulting chromogenic product (formazan precipitate) is proportional to the CK enzymatic activity.
The films are then dried and densitometry is used to quantitate the bands.(Package insert: Sebia Hydragel ISO-CK, Sebia, Norcross, GA)
CK, total: Monday through Sunday; Continuously
CK isoenzymes: Wednesday, Friday; 12 p.m.