Creatine Kinase Isoenzyme Reflex, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Creatine Kinase, Total:
Creatine kinase (CK) is determined by a coupled enzyme reaction where the rate of NADPH formation is measured photometrically and is directly proportional to the CK activity.(Package insert: Roche CK reagent. Indianapolis, IN, July 1999)
Creatine Kinase Isoenzymes:
Electrophoresis is the migration of charge particles in an electrical field. The rate of migration is dependent on such factors as: 1) net electrical charge of the molecule, 2) size and shape of the molecule, 3) strength of the electrical field, 4) properties of the supporting medium, 5) temperature of the operation.
In this procedure, serum CK is separated into the 3 main isoenzymes of CK (MM, MB, and BB) by electrophoretic separation on agarose gel. After electrophoresis, the separated CK isoenzymes are visualized using a chromogenic substrate. The amount of resulting chromogenic product (formazan precipitate) is proportional to the CK enzymatic activity.
The films are then dried and densitometry is used to quantitate the bands.(Package insert: Sebia Hydragel ISO-CK, Sebia, Norcross, GA)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
CK, total: Monday through Sunday; Continuously
CK isoenzymes: Monday through Saturday; 12 p.m.