Chromogranin A, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Chromogranin A (CGA) is measured in a homogeneous automated immunofluorescent assay. This assay uses technology based on a variant of Foerster resonance energy transfer, called time-resolved amplified cryptate emission (TRACE).
A mouse monoclonal antibody against CGA is labeled with europium cryptate (TRACE donor) and a second mouse monoclonal antibody against CGA is labeled with XL665 (TRACE acceptor). CGA is sandwiched between the 2 antibodies, bringing them into close proximity. When the antigen-antibody complex is excited with a nitrogen laser at 337 nm, some fluorescent energy is emitted at 620 nm and the rest is transferred by nonradiative dipole-dipole coupling to XL665. This energy is then emitted as fluorescence at 665 nm. A ratio of the energy emitted at 665 nm to that emitted at 620 nm (internal reference) is calculated for each sample. Signal intensity is proportional to the number of antigen-antibody complexes formed, and therefore to antigen concentration.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 2:30 p.m.