Test ID: HAEVP
Hemolytic Anemia Evaluation
Specimen Type 
Describes the specimen type needed for testing
Whole blood
Whole Blood EDTA
Whole Blood Slide
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
See Recommendations for Collection of Control Specimens for FRAG, HSEP, and HAEVP in Special Instructions.
Specimens must arrive within 96 hours of draw and on Monday through Friday only.
Two whole blood EDTA specimens and 2 whole blood ACD specimens in addition to an EDTA control specimen are required. Include 2 well-made peripheral blood smears, Wright stained or fixed in absolute methanol.
Patient:
Container/Tube: Lavender top (EDTA) and yellow top (ACD [solution B])
Specimen Volume:
EDTA: Two 5-mL vials
ACD (solution B): Two 6-mL vials
Slides: Two peripheral blood smears, Wright stained or fixed in absolute methanol
Collection Instructions:
1. Immediately refrigerate specimens after draw.
2. Do not transfer blood to other containers.
3. Prepare smears.
4. Rubber band patient specimen and control vial together.
Additional Information:
1. Patient's age and sex are required.
2. For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.
Forms:
1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions.
2. Thalassemia/Hemoglobinopathy Information Sheet (Supply T358) is available in Special Instructions
3. If not ordering electronically, please submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.
Normal Control:
Container/Tube: Lavender top (EDTA)
Specimen Volume: 5-mL
Collection Instructions:
1. Draw a control specimen at the same time from a normal, unrelated, non-smoking individual.
2. Clearly write normal control on outermost label and also indicate sex of control on the same label.
3. Immediately refrigerate specimens after draw.
4. Do not transfer blood to other containers.
5. Rubber band control vial and patient specimen together.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross reject |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Control | Refrigerated | 4 days |
| Whole blood | Refrigerated | 4 days |
| Whole Blood EDTA | Refrigerated | 4 days |
| Whole Blood Slide | Refrigerated | |
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