Hemolytic Anemia Evaluation
Specimen Type Describes the specimen type needed for testing
Whole Blood ACD-B
Whole Blood EDTA
Whole Blood Slide
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
See Recommendations for Collection of Control Specimens for FRAG, HSEP, and HAEVP in Special Instructions.
Specimens must arrive within 96 hours of draw and on Monday through Friday only.
Two whole blood EDTA specimens, 2 whole blood ACD specimens, an EDTA control specimen as well as 2 well-made peripheral blood smears (Wright stained or fixed in absolute methanol) are required for testing.
Container/Tube: Lavender top (EDTA) and yellow top (ACD solution B)
EDTA: Two 4-mL vials
ACD (solution B): Two 6-mL vials
Slides: Two peripheral blood smears, Wright stained or fixed in absolute methanol
1. Immediately refrigerate specimens after draw.
2. Do not transfer blood to other containers.
3. Prepare smears.
4. Rubber band patient specimen and control vial together.
1. Patient's age and sex are required.
2. For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.
1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (Supply T576) is available in Special Instructions.
2. Hemolytic Anemia Patient Information Sheet (Supply T705) is available in Special Instructions
3. If not ordering electronically, please submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 5 mL
1. Draw a control specimen at the same time from a normal, unrelated, non-smoking individual.
2. Clearly write normal control on outermost label and also indicate sex of control on the same label.
3. Immediately refrigerate specimens after draw.
4. Do not transfer blood to other containers.
5. Rubber band control vial and patient specimen together.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Whole Blood ACD-B||Refrigerated||4 days|
|Whole Blood EDTA||Refrigerated||4 days|
|Whole Blood Slide||Refrigerated|