Glucose Phosphate Isomerase, Erythrocytes
Method Description Describes how the test is performed and provides a method-specific reference
Washed erythrocytes are hemolyzed and the hemolysate is mixed with glucose, adenosine triphosphate (ATP), glucose-6-phosphate dehydrogenase, and nicotinamide adenine dinucleotide phosphate (NADP). The reduction of NADP is measured spectrophotometrically and is proportional to the enzymatic conversion of ATP and glucose to glucose-6-phosphate.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. Third Edition. New York, Grune and Stratton, 1984, pp 40-42)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday