Method Description Describes how the test is performed and provides a method-specific reference
The Sysmex XE-5000 reticulocyte analyzer is a flow cytometer that uses an argon laser as the light source. Whole blood specimens are stained with polymethine fluorescent dye and passed through the laser beam in a sheath flow cell. Each blood cell causes a forward light scatter that depends on cell size. This measurement permits a separation of the various blood cell types so that only erythrocytes are included in the cell count. Erythrocytes containing the protein reticulin (ie, reticulocytes) are stained by the dye in the laser beam. The right-angle fluorescence is measured to count these stained cells. The instrument counts 30,000 erythrocytes in each specimen. Because a known aliquot is counted, an actual RBC per unit volume is performed and an absolute reticulocyte count is obtained.(Operatorâ€™s manual: Sysmex Corporation. Automated Hematology Analyzer XE-5000 Main Unit, North American Edition. Code No. 461-2638-6. October 2006; Kojma K, Niri M, Setoguchi K, et al: An automated optoelectronic reticulocyte counter. Am J Clin Pathol 1989;92:57-61)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously