Plasminogen Activity, Plasma
Method Description Describes how the test is performed and provides a method-specific reference
This assay is performed using the HemosIL Plasminogen Kit on the Beckman Coulter ACL TOP. The method is an automated chromogenic assay in which an excess of streptokinase (SK) in the presence of fibrinogen is added to sample plasma containing plasminogen. A plasminogen-streptokinase complex is formed. The complex catalyzes the splitting of p-nitroaniline (pNA) from the substrate S-2403 pyroGlu-Phe-Lys-pNAHCl. Under these conditions the enzymatic activity of the complex is not inhibited by plasma inhibitors. The rate at which the pNA is released is measured kinetically at 405 nm and is directly proportional to the plasminogen level in the test specimen. The concentration of plasminogen is calculated from a standard curve prepared from reference plasma dilutions.(Friberger P, Knos M: Plasminogen determination in human plasma. In Chromogenic Peptide Substrates: Chemistry and Clinical Usage. Edited by Edinburgh. Churchill Livingstone, 1979, pp 128-140)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday