Nucleophosmin (NPM1) Mutation Analysis
Method Description Describes how the test is performed and provides a method-specific reference
DNA is extracted from blood or bone marrow and a PCR assay is performed using primers that amplify a fragment of NPM1 DNA containing the region susceptible to insertion mutation. One of the PCR primers contains a fluorescent tag. The amplified fragments are separated by capillary electrophoresis on an ABI3130 genetic analyzer. In unmutated DNA, the PCR fragments obtained are 187 base pairs (bp). PCR fragments containing an insertional mutation are longer, usually 191 bp, as the majority of mutations are 4 bp insertions.(Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday: 3 p.m.