Chromosome Analysis, CpG Mitogen Study for B-Cell Disorder
Method Description Describes how the test is performed and provides a method-specific reference
A portion of the whole blood or bone marrow is transferred to flasks containing media and CpG 7909 (CpG) mitogen or media without mitogens. The cells are incubated for 24 to 72 hours at 37 degrees C. In the harvest process, the cells are exposed to colcemid and hypotonic solution, then fixed with glacial acetic acid and methanol. Metaphase cells are dropped onto microscope slides and stained with quinacrine or Leishman stain. Twenty metaphases (10 metaphases from cultures with CpG and 10 metaphases from cultures without mitogens) are usually examined for structure and number of chromosomes. If a clone is suspected, but not confirmed within 20 metaphases, 30 metaphases may be analyzed. Minimal evidence for the presence of an abnormal clone is defined as 2 or more metaphases with the same structural abnormality or chromosome gain (trisomy), or 3 or more metaphases lacking the same chromosome. All metaphases are captured using a computerized imaging system, and 1 or more karyograms from each clone are prepared to document the type of abnormality and to permit systematic interpretation of the abnormalities.(Unpublished Mayo method)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday.
Results reported Monday through Friday; 8 a.m. to 5 p.m. CST.