B-Cell Lymphoma, FISH, Blood or Bone Marrow
Method Description Describes how the test is performed and provides a method-specific reference
Anomalies involving BCL6, c-MYC, and IGL are detected using a break-apart FISH probe. If a MYC abnormality is detected, reflex testing will be performed to identify the translocation partner. Translocations involving fusion of CCND1 and IGH; BCL2 and IGH; MYC and IGH; BIRC3 and MALT1; IGH and MALT1; and MYC and IGL are detected using dual-color, dual-fusion (D-FISH) strategies. A total of 200 nuclei are scored for the break-apart probe set. For each D-FISH probe set, 500 interphase nuclei are scored and results for each abnormal probe(s) are expressed as percent abnormal nuclei.(Brockman SR, Paternoster SF, Ketterling RP, et al: New highly sensitive fluorescence in situ hybridization method to detect PML/RARA fusion in acute promyelocytic leukemia. Cancer Genet Cytogenet 2003;145:144-51)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 6 a.m.-9 p.m., Saturday, Sunday; 6 a.m.-4 p.m.