T-Cell Acute Lymphoblastic Leukemia (T-ALL), FISH
Method Description Describes how the test is performed and provides a method-specific reference
Identification of deletions of CDKN2A and TP53 are detected using FISH enumeration strategy probes. Rearrangements of STIL/TAL1, TRB, MLL, and TRAD are detected using dual-color, break-apart (BAP) strategy probes. Dual-color, dual-fusion (D-FISH) strategy probe sets are used to detect t(5;14)(q35;q32), t(9;22)(q34;q11.2), t(10;11)(p13;q14), and in reflex testing when rearrangements of MLL, TRB, or TRAD genes are detected. Amplification of the ABL1 gene on chromosome band 9q34 is detected using a D-FISH probe strategy. For the enumeration and BAP strategy probe sets 200 interphase nuclei are scored and 500 interphase nuclei are scored when D-FISH probes are used. Two technologists analyze each probe set and all results are expressed as percent abnormal nuclei.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Specimens are processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.