Test ID: VWFM2
von Willebrand Factor Multimer Analysis, Plasma
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Platelet-poor plasma proteins are denatured using heat and an anionic detergent, sodium dodecyl sulfate. The sample is then electrophoresed through a discontinuous agarose gel on a cooled horizontal electrophoresis unit overnight to separate the von Willebrand factor (VWF) multimers by size. The gel is fixed in acid and isopropanol, washed in water, and incubated with dilute rabbit antihuman VWF. After washing away unbound antibody, the gel is incubated with dilute goat antirabbit IgG antibody tagged with an infrared dye. Excess secondary antibody is washed away, and the gel is scanned using an infrared imaging system. The digitized image of the electrophoretic distribution of the VWF multimers is interpreted by a coagulation consultant and a written report is provided.(Ruggeri ZM, Zimmerman TS: Variant von Willebrand's disease. Characterization of two subtypes by analysis of multimeric composition of FVIII/vWF in plasma and platelets. J Clin Invest 1980;65:1318-1325)
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