von Willebrand Factor Multimer Analysis, Plasma
Method Description Describes how the test is performed and provides a method-specific reference
Platelet-poor plasma proteins are denatured using heat and an anionic detergent, sodium dodecyl sulfate. The sample is then electrophoresed through a discontinuous agarose gel on a cooled horizontal electrophoresis unit overnight to separate the von Willebrand factor (VvWF) multimers by size. The gel is fixed in acid and isopropanol, washed in water, and incubated with dilute rabbit antihuman VvWF. After washing away unbound antibody, the gel is incubated with dilute goat antirabbit IgG antibody tagged with an infrared dye. Excess secondary antibody is washed away, and the gel is scanned using an infrared imaging system. The digitized image of the electrophoretic distribution of the vWF multimers is interpreted by a coagulation consultant and a written report is provided. (Ruggeri ZM, Zimmerman TS:, Variant von Willebrand's disease. Characterization of two subtypes by analysis of multimeric composition of FVIII/vWF in plasma and platelets. J Clin Invest 1980;65:1318-1325)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday, Tuesday; 9 a.m.-4 p.m.