Imatinib Mesylate Responsive Genes, Locus Anomalies, FISH
Method Description Describes how the test is performed and provides a method-specific reference
Identification of rearrangements of 1q25 (ABL2), 9q34 (ABL1), 5q33 (PDGFRB), and 4q12 (CHIC2 - FIP1L1 and PRGFRA fusion result in a loss of the CHIC2 locus), is performed using FISH break-apart strategy. For each probe set, 200 interphase nuclei are scored. Results for each abnormal probe is expressed as percent abnormal nuclei. (Brockman SR, Paternoster SF, Ketterling RP, et al: New highly sensitive fluorescence in situ hybridization method to detect PML/RARA fusion in acute promyelocytic leukemia. Cancer Genet Cytogenet 2003;145:144-151)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.