Acute Myeloid Leukemia (AML), FISH
Method Description Describes how the test is performed and provides a method-specific reference
Identification of deletions or monosomy of chromosomes 5, 7, 13, trisomy of chromosome 8 and deletions or rearrangements of chromosome 17 and 20 are detected based on FISH enumeration strategy probes. Rearrangements involving MLL are detected using a dual-color break-apart (BAP) strategy probe. Dual-color dual-fusion (D-FISH) strategy probe sets are used to detect inv(3) MYH11/CBFB, inv(16) RPN1/MECOM, t(8;21), t(15;17), t(6;9), t(8:16), t(3:21), t(1;3), t(11;22), t(9;22), and in reflex testing when rearrangements of the MLL gene are detected. For enumeration and BAP strategy probe sets 200 interphase nuclei are scored; 500 interphase nuclei are scored when D-FISH probes are used. Two technologists analyze each probe set and all results are expressed as percent abnormal nuclei.(Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.