Beta-2 Glycoprotein 1 Antibodies, IgG, Serum
Method Description Describes how the test is performed and provides a method-specific reference
This indirect, noncompetitive enzyme-linked immunosorbent assay (ELISA) uses microtiter plates coated with purified, human beta 2 glycoprotein 1 (beta 2 GP1). Beta 2 GP1 antibodies bound in the first stage of the assay are detected with antihuman IgG horseradish peroxidase antibody conjugate in the second stage of the assay by adding 3,3',5,5' tetramethylbenzidine substrate (10 minute incubation followed by addition of 0.5 M H2SO4). The absorbance of test sera is compared to a single cutoff calibrator. Results for test sera are expressed in arbitrary U/mL by comparison with standard curves obtained using calibrators at 0 U/mL, 4 U/mL, 8 U/mL, 20 U/mL, 50 U/mL, and 100 U/mL. (Package insert: Varelisa Beta 2 Glycoprotein 1 IgM, Antibodies, doc 187-01, Phadia Diagnostics, Portage, MI)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 8 a.m.