Test ID: PLAB
Platelet Antibody, Serum
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Solid-phase enzyme immunoassay (SPEIA) uses purified platelet membrane glycoproteins as "targets" rather than intact platelets and this provides a simple "panel" of target antigens. These purified glycoproteins are permanently affixed to the interior of concave wells in plastic trays. The attachment of patient antibodies to these glycoproteins is detected by the addition of a second stage antihuman globulin reagent which is enzyme tagged. The addition of enzyme substrate is followed by a visible color change which permits rapid visual reading of the tray or automatable reading.(Moore SB, DeGoey SR: Serum platelet antibody testing: evaluation of solid-phase enzyme immunoassay and comparison with indirect immunofluorescence. Am J Clin Pathol 1998;109:190-195)
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