Mucopolysaccharides (MPS) Screen, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Centrifuged urine is mixed with a dimethylmethylene blue (DMB)/Tris hydroxymethlyaminomethane (TRIS) solution. The metachromatic dye DMB binds with glycosaminoglycans (GAG) and the complex formed changes the spectra of the dye. The pink color complex is measured at 520 nm.(deJong JG, Wevers RA, Liebrand-van Sambeek R: Measuring urinary glycosaminoglycans in the presence of protein; an improved screening procedure for mucopolysaccharidoses based on dimethylmethylene blue. Clin Chem 1992;38:803-807; Panin G, Naia S, Dall'Amico R, et al: Simple spectrophotometric quantification of urinary excretion of glycosaminoglycan sulfates. Clin Chem 1986;32:2073-2076)
Thin-Layer Chromatography (TLC):
Following precipitation, urinary GAG is separated by TLC based on the solubility of their calcium salts in varying ethanol concentration solvents. After separation, the sulfates are stained with Alcian blue for visualization.(Dembrune PP, Roesel RA: Screening for mucopolysaccharidoses by analysis of urinary glycosaminoglycans. In Techniques in Diagnostic Human Biochemical genetics-A Laboratory Manual. Edited by FA Hommes. New York, Wiley-Liss, 1991, pp 77-86)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
MPS, quantitative: Monday through Friday; 12 p.m.
MPS, qualitative: Monday; 8 a.m.