PDGFRB/TEL Translocation (5;12) for Chronic Myelomonocytic Leukemia, FISH
Method Description Describes how the test is performed and provides a method-specific reference
This test uses a Mayo-validated probe set to detect the t(5;12)(q33;p13) by fluorescence in situ hybridization (FISH). Blood or bone marrow is processed into fixed cell pellets according to standard culture room techniques. One slide is processed according to standard FISH procedures and probed with the PDGFRB/ETV6 probe set. The PDGFRB/ETV6 probe set uses a double (D)-FISH strategy to detect the 5;12 translocation. Abnormal nuclei have 1 red signal (1R), 1 green signal (1G), and 2 yellow (2Y; fusion) signals, while normal nuclei should have a 2R and 2G pattern, though other abnormal patterns may be observed (ie, 3R2G and 2R2G1Y). After hybridization and washing, 2 technologists each score 250 (500 total) nuclei and the results are expressed as a percent abnormal nuclei. (Brockman SR, Paternoster SF, Ketterling RP, et al: New highly sensitive fluorescence in situ hybridization method to detect PML/RARA fusion in acute promyelocytic leukemia. Cancer Genetics & Cytogenetics 2003;145:144-151)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.