Uric Acid, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Uric acid is oxidized by uricase to form allantoin and hydrogen peroxide. The hydrogen peroxide reacts with TOOS (N-ethyl-N-(2- hydroxy-3-sulfopropyl)-3-mehtylaniline) and 4-aminophenazone in the presence of peroxidase to form a quinoneimine dye. The intensity of the red color formed is proportional to the uric acid concentration. Prior to the start of the reaction, the sample is initially incubated with a reagent mixture containing ascorbate oxidase and a clearing system. This eliminates any ascorbic acid present in the sample which could interfere with the peroxidase indicator reaction. (Package insert: Roche Uric Acid reagent, Roche Diagnostic Corp., Indianapolis IN, 1998)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously