CHIC2, 4q12 Deletion (FIP1L1 and PDGFRA Fusion), FISH
Method Description Describes how the test is performed and provides a method-specific reference
CHIC2 deletion studies can be performed on 1 mL of bone marrow aspirate or 10 mL of blood drawn into sodium heparin. DNA probes that map to FIP1L1, CHIC2, and PDGFRA at 4q12, hybridize to the chromosomes of cells from bone marrow or peripheral blood. These probes are visualized by fluorescent microscopy. Normal metaphase and interphase cells have 2 green/red/aqua fusion signals signifying the hybridization of the F1P1L1, CHIC2 and PDGFRA probes, respectively, to both chromosomes 4 at band 4q12. Cells with del(4)(q12) have 1 green/aqua fusion signal from the deleted chromosome 4 deletion of CHIC2) and 1 red/green/aqua fusion signal from the normal chromosome 4 at 4q12. Cells with ins(var;4)(var;q12) have 1 green/aqua fusion from the abnormal chromosome 4, 1 red on the insertional translocation recipient chromosome and 1 green/red/aqua fusion signal from the normal chromosome 4 at 4q12. Cells with translocations involving PDGRFA have 1 red/green fusion signal on the abnormal chromosome 4, 1 aqua signal on the translocation recipient chromosome and 1 green/red/aqua signal on the normal chromosome 4.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.