Cholesterol, HDL, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Sulfated alpha-cyclodextrin forms water soluble complexes selectively with low density lipoprotein, very low density lipoprotein, and chylomicrons, and renders these lipoprotein particles resistant to the enzymatic activity of polyethylene glycol (PEG)-modified cholesterol esterase and cholesterol oxidase. PEG-modified cholesterol esterase selectively converts high density lipoprotein (HDL) cholesterol esters to free cholesterol, which, in the presence of O(2) reacts with PEG-cholesterol oxidase to produce delta-cholestenone and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide generated reacts with 4-aminophenazone and N-ethyl-N(3 methylphenyl)-N-succinyl ethylene diamine to form a quinone amine dye. The color intensity of this dye, measured photometrically at 600 nm, is proportional to the HDL-cholesterol concentration in the specimen. This method is referenced to the Centers of Disease Control and Prevention (CDC) standardized method performed in the Cardiovascular Risk Assessment Laboratory. (Package insert: Roche HDL-C Reagent, Roche Diagnostic Corp., Indianapolis, October 1999)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously