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Test ID: HDCH    
Cholesterol, HDL, Serum

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Method Description Describes how the test is performed and provides a method-specific reference

Sulfated alpha-cyclodextrin forms water soluble complexes selectively with low density lipoprotein, very low density lipoprotein, and chylomicrons, and renders these lipoprotein particles resistant to the enzymatic activity of polyethylene glycol (PEG)-modified cholesterol esterase and cholesterol oxidase. PEG-modified cholesterol esterase selectively converts high density lipoprotein (HDL) cholesterol esters to free cholesterol, which, in the presence of O(2) reacts with PEG-cholesterol oxidase to produce delta-cholestenone and hydrogen peroxide. In the presence of peroxidase, the hydrogen peroxide generated reacts with 4-aminophenazone and N-ethyl-N(3 methylphenyl)-N-succinyl ethylene diamine to form a quinone amine dye. The color intensity of this dye, measured photometrically at 600 nm, is proportional to the HDL-cholesterol concentration in the specimen. This method is referenced to the Centers of Disease Control and Prevention (CDC) standardized method performed in the Cardiovascular Risk Assessment Laboratory. (Package insert: Roche HDL-C Reagent, Roche Diagnostic Corp., Indianapolis, October 1999)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location The location of the laboratory that performs the test

Rochester