ZAP-70, Chronic Lymphocytic Leukemia (CLL) Prognosis
Method Description Describes how the test is performed and provides a method-specific reference
Ficoll-separated cells are initially stained with CD3 and CD19, then treated with saponin and incubated with ZAP-70 antibody. Flow cytometry is used to determine the percent of B cells positive for ZAP-70. Staining is also performed on a normal peripheral blood control specimen. The threshold for ZAP-70 staining is established by using normal B cells as the negative cutoff value and comparing with background-positive T-cell staining.(Rassenti LZ, Huynh L, Toy TL, et al: ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. N Engl J Med 2004 August 26;351:893-901)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 9 a.m.