Amylase, Total, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The liquid Roche amylase (AMYL) method is an enzymatic colorimetric test using 4,6-ethyliden (G7)-p-nitrophenol (G1)-a, D-maltoheptaoside (ethylidene-G7PNP) as a substrate. Human salivary and pancreatic amylase convert the substrate at approximately the same rate. The a-amylase cleaves the substrate into G2, G3, G4 PNP fragments. The G2 and G3 and G4 PNP fragments are further hydrolyzed by an a-glucosidase to yield p-nitrophenol and glucose. The rate of increase in absorbance at 415 nm (measuring the increase in p-nitrophenol) is proportional to amylase activity.(Package insert: Roche AMYL reagent, Indianapolis, IN, February 2000)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously