Test ID: BA190
BCR/ABL, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay
‹ Back to Hematology index
Total RNA is extracted from the sample using the QIAmp kit (Qiagen). cDNA is made using SuperScript III Kit (Invitrogen). A quantitative, real-time-PCR reaction is performed using the LightCycler 2.0 instrument (Roche) with one set of primers designed to detect the bcr/abl e1/e2 fusion and a second set of primers designed to detect a fragment of abl. Taqman-type probe technology is used in both reactions. The data is analyzed using the supplied software for relative quantification with calibrator normalization and efficiency correction. (Roche Applied Science LightCycler 2.0 Instrument Operation Manual, Version 4/1.2. 2003). The abl is amplified to control for RNA degradation in the sample and the calibrator is used to control for inter-run variations. A normalized ratio of bcr/abl(p190) mRNA:abl mRNA is obtained and converted to the reported value of percent bcr/abl(p190):abl.(Unpublished Mayo method)
Monday through Friday a.m.
RNA: Up to 3 months