Cholesteryl Esters, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Unesterified (free) cholesterol is measured by direct analysis of serum using reagents supplied by Wako Chemicals USA, Inc., as adapted to an automated chemistry analyzer. Free cholesterol reacts with cholesterol oxidase to generate hydrogen peroxide which reacts with 3,5-dimethoxy-N-(2-hydroxy-3-sulfopropyl) aniline sodium (DAOS) and 4-aminoantipyrene to produce a blue pigment. The product is proportional to the serum free cholesterol concentration. The result is expressed as percent esterified cholesterol based on the total serum cholesterol concentration obtained by conventional assay.(Allain CC, Poon LS, Chan CSG, et al: Enzymatic determination of total serum cholesterol. Clin Chem 1974;20:470-475; package insert: Wako Free Cholesterol E. Wako Chemicals USA, Inc., Richmond, VA)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 7 a.m.-5 p.m.