Chronic Lymphocytic Leukemia (CLL), FISH
Method Description Describes how the test is performed and provides a method-specific reference
The specimen is first processed using standard cytogenetic procedures. This assay uses DNA probes to detect common chromosome abnormalities in nonproliferating nuclei (interphase cells) in B-cell chronic lymphocytic leukemia (B-CLL). The loci tested include 6cen (D6Z1), 6q23 (MYB), 11cen (D11Z1), 11q13 (CCND1), 11q23 (ATM), 12cen (D12Z3), 12q15 (MDM2), 13q14 (D13S319), 13qter (D13S327), 14q32 (IgH), 17cen (D17Z1), and 17p13.1 (TP53). A total of 200 interphase nuclei are scored for each probe and the results are expressed as percent abnormal nuclei. If the results show an abnormality of IGH other than fusion with CCND1 then the specimen is processed with FISH to detect fusion of IGH with BCL2 (18q21), and IGH with BCL3 (19q13.3). (Dyer MJ, Oscier DG: The configuration of the immunoglobulin genes in B cell chronic lymphocytic leukemia. Leukemia 2002 Jun;16:973-984)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.