Therapeutic Antibody by Flow Cytometry
Method Description Describes how the test is performed and provides a method-specific reference
Flow cytometric immunophenotyping of peripheral blood, bone marrow, or tissue-derived lymphocytes is performed to assess the expression of the cell-surface antigen corresponding to the monoclonal antibody therapeutic target. The following antibody panels will be used:
-Anti-CD20 assessment: kappa/lambda/CD19/CD20
-Anti-CD25 assessment: CD25/CD19/CD3/CD45
-Anti-CD49d assessment: CD19/CD49d/CD3/CD45
-Anti-CD52 assessment: CD52/CD19/CD3/CD45
(Flow Cytometry in Clinical Diagnosis. Fourth Edition. Edited by P Keren, JP McCoy Jr, J Carey. ASCP Press, Chicago, IL, 2007)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Specimens are processed Monday through Saturday and reported Monday through Friday.