Phospholipid (Cardiolipin) Antibodies, IgG and IgM, Serum
Method Description Describes how the test is performed and provides a method-specific reference
This test uses microtiter plates coated with bovine cardiolipin and purified bovine beta 2 glycoprotein 1(beta 2 GP1). Phospholipid antibodies bound in the first stage of the assay are detected with antihuman horseradish peroxidase antibody conjugate in the second stage of the assay by adding 3,3',5,5' tetramethylbenzidine substrate (10 minute incubation followed by addition of 0.5 M HSO). Results for test sera are expressed in U/mL by comparison with standard curves obtained using calibrators at 0, 4, 8, 20, 40 and 100 units.(Package Insert: Varelisa Cardiolipin IgG, Antibodies, Pharmacia Diagnostics, 157-01)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 4 p.m.