Electrophoresis, Protein, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Urine proteins are separated in an electric field according to their size, shape, and electric charge (Helena SPIFE 3000). The separation is performed on agarose gels (Helena SPIFE SPE Vis Gel). The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry (Helena Quick Scan 2000). Multiplying by the urine protein concentration (Coomasie blue) converts the percentage of protein in each fraction into urine concentration. (Instruction manual: Helena SPIFE 3000; package insert: Helena SPIFE SPE Vis Gel, 2001; Kyle RA, Katzmann JA, Lust JA, Dispenzieri A: Clinical indications and applications of electrophoresis and immunofixation. In Manual of Clinical Laboratory Immunology. 6th edition. Edited by NR Rose, RG Hamilton, B Detrick: Washington, DC, ASM Press, 2002 pp 71-91)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Protein, total: Monday through Sunday; Continuous
Electrophoresis, protein: Monday through Saturday; 12:00 p.m.