Hemoglobin A1c, Blood
Method Description Describes how the test is performed and provides a method-specific reference
The BIO-RAD Variant II Turbo Hemoglobin A1c Program utilizes principles of ion-exchange HPLC. The samples are automatically diluted on the Variant II Turbo Sampling Station (VSS) and injected into the analytical cartridge. The Variant II Turbo Chromatographic Station (VCS) dual pumps deliver a programmed buffer gradient of increasing ionic strength to the cartridge, where the hemoglobins are separated based on their ionic interactions with the cartridge material. The absorbance at 415 nm is measured. An additional filter at 690 nm corrects the background absorbances. The Trinity Biotech ultra-2, a boronate-affinity chromatography method, is used when the Variant II Turbo does not produce reliable results. Both methods are certified by National Glycohemoglobin Standardization Program as having documented traceability to the DCCT reference method.(Instruction manual: Bio-Rad Variant II Turbo Hemoglobin A1c Program, L70245800, Hercules, CA)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously