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Test ID: HBA1C    
Hemoglobin A1c, Blood

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Method Description Describes how the test is performed and provides a method-specific reference

The BIO-RAD Variant II Turbo Hemoglobin A1c Program utilizes principles of ion-exchange HPLC. The samples are automatically diluted on the Variant II Turbo Sampling Station (VSS) and injected into the analytical cartridge. The Variant II Turbo Chromatographic Station (VCS) dual pumps deliver a programmed buffer gradient of increasing ionic strength to the cartridge, where the hemoglobins are separated based on their ionic interactions with the cartridge material. The absorbance at 415 nm is measured. An additional filter at 690 nm corrects the background absorbances. The Bio-Rad in2it, a boronate-affinity chromatography method, is used when the Variant II Turbo does not produce reliable results. Both methods are certified by National Glycohemoglobin Standardization Program as having documented traceability to the DCCT reference method.(Instruction Manual: Bio-Rad Variant II Turbo Hemoglobin A1c Program, L70245800, Hercules, CA, March 2006)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester