von Willebrand Disease 2N (Subtype Normandy), Blood
Method Description Describes how the test is performed and provides a method-specific reference
Direct mutation analysis: Based on the PCR, 3 regions of the von Willebrand factor gene (exons 18, 19, 20) are individually amplified from leukocyte genomic DNA. Each amplification product is restricted by an endonuclease (exon 18: Tai I [Thr791Met]; exon 19: Ava II [Arg816Trp]; exon 20: Msp I [Arg854Gln]) followed by agarose-gel electrophoresis and ethidium bromide staining of the restriction products. A Polaroid picture records information to be interpreted. (Molecular cloning: a laboratory manual. 2nd edition. Edited by J Sambrook, EF Fritsch, T Maniatis. Cold Spring Harbor, NY, Cold Spring Harbor Press, 1989, p 53)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday; 1 p.m.