B-Cell Acute Lymphoblastic Leukemia (ALL), FISH
Method Description Describes how the test is performed and provides a method-specific reference
Specimens are processed in the cytogenetic laboratory using standard cytogenetic procedures. This method uses DNA probes to detect common chromosome anomalies in interphase cells from patients with ALL. The loci tested include 1q23 (PBX1), 4cen (D4Z1), 9p21 (CDKN2A), 9cen (D9Z1), 9q34 (ABL1), 10cen (D10Z1), 11q23 (MLL), 12p13 (ETV6), 17cen (D17Z1), 19p13.3 (TCF3), 21q11.2 (RUNX1), 14q32 (IGH), and 22q11.2 (BCR). A total of 500 interphase nuclei are scored for each D-FISH probe set and 200 interphase nuclei for each ND-FISH and break-apart probe (BAP)-FISH probe set. The results are expressed as percent abnormal nuclei. (Dewald GW: Unpublished Mayo information)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.