11 Beta-Prostaglandin F2 Alpha, Urine
Method Description Describes how the test is performed and provides a method-specific reference
11 Beta-prostaglandin F2 alpha, linked to an Acetylcholinesterase tracer, and endogenous 11 beta-prostaglandin F2 alpha compete for a limited number of rabbit antibody binding sites. The antibody-bound antigen binds to an anti-rabbit IgG attached to the well. The unbound antigen is then washed away, and Ellman's reagent is added to each well. The enzyme reaction between the bound acetylcholinesterase and the Ellman's reagent produces a yellow color that absorbs at 412 nm. The absorbance is inversely proportional to the amount of free 11 beta-prostaglandin F2 alpha. (Package insert: 11 Beta-prostaglandin F(2) Alpha Enzyme Immunoassay Kit, Cayman Chemical Company, Ann Arbor, MI)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday, Thursday; 10:00 a.m.