JC Virus (JCV) Detection by In Situ Hybridization
Method Description Describes how the test is performed and provides a method-specific reference
The probes are labeled with digoxigenin and used in an in situ hybridization procedure with hybridization for 2 hours. After washing, the tissues are incubated with anti-digoxigenin antibody conjugated to alkaline phosphatase and the hybridization product is detected by reaction of the enzyme conjugate with the substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and concomitant reduction of nitroblue tetrazolium (NBT). Some bizarre, malignant-appearing astrocytes may stain with the JCV probe. These cells are infected by JCV in progressive multifocal leukoencephalopathy (PML). Morphologically, these cells have enlarged nuclei (25-60 nm), lobulated nuclei, multiple nuclei, nuclear hyperchromasia with a coarse chromatin pattern, and occasional frank mitosis. (Aksamit AJ, Mourrain P, Sever JL, Major EO: Progressive multifocal leukoencephalopathy: investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol 1985;18:490-496)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 9 a.m.