Erythropoietin (EPO), Serum
Method Description Describes how the test is performed and provides a method-specific reference
Testing is performed on the Beckman Coulter DxI 800. The Access erythropoietin (EPO) assay is a 2-site immunoenzymatic "sandwich" assay. A sample is added to a reaction vessel along with the paramagnetic particles coated with mouse monoclonal anti-EPO, blocking reagent and the alkaline phosphatase conjugate. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of EPO in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve. (Instruction manual: Beckman Coulter Access EPO, Beckman Coulter Access Immunoassay Systems, Beckman Coulter, Inc, Fullerton CA 2009)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.