B-Cell Lymphoma, FISH, Tissue
Method Description Describes how the test is performed and provides a method-specific reference
This test is performed using either commercially available or laboratory-developed probes. Rearrangements (MYC, BCL2, BCL6, CCND2, or MALT1) are detected using dual-color break-apart (BAP) strategy probes, translocations (involving MYC, MALT1, or CCND1/IGH) are identified using dual-color, dual-fusion (D-FISH) strategy probes, and deletions (7q32 or TP53) using enumeration strategy probes. Formalin-fixed, paraffin-embedded tissues are cut at 5 microns and mounted on positively charged glass slides. The selection of tissue and the target areas on the hematoxylin and eosin (H and E)-stained slide is performed by a pathologist. Using the H and E-stained slide as a reference, target areas are etched with a diamond-tipped etcher on the back of the unstained slide to be assayed. The probe set is hybridized to the appropriate target areas and 2 technologists each analyze 100 interphase nuclei each (200 total) with the results expressed as a percent abnormal nuclei.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday 8 a.m.-5 p.m., CST.