Leukemia/Lymphoma Immunophenotyping by Flow Cytometry, Tissue
Method Description Describes how the test is performed and provides a method-specific reference
Flow cytometric immunophenotyping of tissues is performed using the following antibodies:
Tissue Panel: CD2, CD3, CD5, CD7, CD10, CD19, CD20, CD23, CD45, 7-AAD, and kappa and lambda light chains.
Possible additional panels:
B-cell Panelâ€“CD5, CD10, CD11c, CD19, CD20, CD22, CD38, CD45, CD103 and kappa and lambda light chains
T-cell Panelâ€“CD2, CD3, CD4, CD5, CD7, CD8, and gamma/delta
KIR Panelâ€“CD3, CD8, CD16, CD56, CD57, CD94, CD158a, CD158b, CD158e (p70) and NKG2a
Acute Panelâ€“CD2, CD3, CD5, CD7, CD10, CD13, CD15, CD19, CD20, CD33, CD34, CD45, CD56, CD117 and HLA-DR
MPO/TdT Panelâ€“cytoplasmic CD3, CD13, cytoplasmic CD22, CD34, CD45, cytoplasmic CD79a, nuclear TDT and cytoplasmic MPO.
Plasma Cell Panelâ€“CD19, CD38, CD45, CD138 and cytoplasmic kappa and lambda light chains
(Stetler-Stevenson M, Ahmad E, Barnett D, et al: Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells: Approved Guideline-Second Edition. CLSI document H43-A2 2007. CLSI, Wayne, PA)
Supplemental Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Specimens are processed and reported Monday through Saturday