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Test ID: FAML    
Acute Myeloid Leukemia (AML), FISH

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NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Useful For Suggests clinical disorders or settings where the test may be helpful

Detecting a neoplastic clone associated with the common chromosome anomalies seen in patients with acute myeloid leukemia or other myeloid malignancies

 

Evaluating specimens in which standard cytogenetic analysis is unsuccessful

 

Identifying and tracking known chromosome anomalies in patients with myeloid malignancies and tracking response to therapy

Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
ADD1FOne Additional FISH ProbeNoNo
ADD2FTwo Additional FISH ProbesNoNo
ADD4FFour Additional FISH ProbesNoNo
ADD3FThree Additional FISH ProbesNoNo
ADD5FFive Additional FISH ProbesNoNo
ADD6FSix Additional FISH ProbesNoNo
ADD7FSeven Additional FISH ProbesNoNo
ADD8FEight Additional FISH ProbesNoNo
ADD9FNine Additional FISH ProbesNoNo
ADD10Ten Additional FISH ProbesNoNo
ADD11Eleven Additional FISH ProbesNoNo
ADD12Twelve Additional FISH ProbesNoNo
14FPFourteen Additional FISH ProbesNoNo
13FPThirteen Additional FISH ProbesNoNo
15FPFifteen Additional FISH ProbesNoNo
16FPSixteen Additional FISH ProbesNoNo
17FPSeventeen Additional FISH ProbesNoNo
18FPEighteen Additional FISH ProbesNoNo
19FPNineteen Additional FISH ProbesNoNo
20FPTwenty Additional FISH ProbesNoNo
21FPTwenty One Additional FISH ProbesNoNo
22FPTwenty Two Additional FISH ProbesNoNo
23FPTwenty Three Additional FISH ProbesNoNo
24FPTwenty Four Additional FISH ProbesNoNo
25FPTwenty Five Additional FISH ProbesNoNo
26FPTwenty Six Additional FISH ProbesNoNo
27FPTwenty Seven Additional FISH ProbesNoNo
28FPTwenty Eight Additional FISH ProbesNoNo
29FPTwenty Nine Additional FISH ProbesNoNo
30FPThirty Additional FISH ProbesNoNo

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

See Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up in Special Instructions.

 

When this test is ordered, a charge for 2 FISH probes and interpretation is included. If additional probes or the entire panel are ordered, additional probe charges will be added.

 

Please indicate if the entire panel is to be performed. This is suggested for patients with a new diagnosis of acute myeloid leukemia. If the patient is being treated for known anomalies, indicate which anomalies need to be investigated.

 

Indicate subtype as well as which anomalies need to be investigated from the following profile:

t(8:21), [M2], RUNX1T1/RUNX1

t(15:17), [M3], PML/RARA

t(11q23:var), [M0-M7], MLL

inv(16), [M4, Eos], MYH11/CBFB

+8, [M0-M7], Cen8/MYC

t(6;9), [M2, M4], DEK/NUP214

inv(3), [M1, M2, M4, M6, M7], RPN1/MECOM

t(8;16), [M4, M5], MYST3/CREBBP

t(3;5), [M2, M4, M6], MLF1/NPM1

t(1;22), [M7], RBM15/MKL1*

-5/5q-, D5S630/EGR1

-7/7q-, D7S486/Cen7

13q-, D13S319/LAMP1

17p-, TP53/Cen17

20q-, D20S108/20qter

t(9;22), BCR/ABL1

 

*The RBM15/MKL1 probe set will only be used to test patients with a suspected or confirmed diagnosis of M7 or to confirm a t(1;22) identified by chromosome analysis.

-When an MLL rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(6;11)(q27;q23) MLLT4/MLL, t(9;11)(p22;q23) MLLT3/MLL, t(10;11)(p13;q23) MLLT10/MLL, t(11;16)(q23;p13.3) MLL/CREBBP, t(11;19)(q23;p13.1) MLL/ELL, or t(11;19)(q23;p13.3) MLL/MLLT1.

-When 3 copies of MECOM are observed with no fusion with RPN1, reflex testing using the MECOM/RUNX1 probe set is performed to identify a potential t(3;21)(q26.2;q22) rearrangement.

-When 3 copies of RPN1 are observed with no fusion with MECOM, reflex testing using the PRDM16/RPN1 probe set is performed to identify a potential t(1;3)(p36;q21).

 

This assay detects chromosome abnormalities observed in the blood and bone marrow of patients with acute myeloid leukemia. For testing paraffin-embedded tissue samples from patients with myeloid sarcoma, see FMST / Myeloid Sarcoma, FISH, Tissue.

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Fluorescence In Situ Hybridization (FISH)

Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

AML, FISH

Aliases Lists additional common names for a test, as an aid in searching

-5
-7
11q23
5q-
7q-
Acute Myeloid Leukemia (AML)
Acute Promyelocytic Leukemia (APL)
AF6/MLL
AF9/MLL
AML-M2
AML-M3
AML-M4
AML-M4eo
AML-M5
APL (Acute Promyelocytic Leukemia)
DEK/CAN
Deletion 5q
Deletion 7q
ETO/AML1
EVI1/AML1
FISH (Fluorescence In Situ Hybridization)
Inv(16)
Inv(3)
inversion 16
inversion 3
MLL
MYH11/CBFb
NUP214
PML/RARA
t(15:17)
t(16:16)
t(3:21)
t(3;3)
t(6;9)
t(8:16)
t(8;21)
t(9;11)
RUNX1T1/RUNX1
MECOM/RUNX1
KAT6A/CREBBP
+8
13q-
17p-
20q-
AF10/MLL
AML-M0
AML-M1
AML-M7
BCR/ABL
BCR/ABL1
DEK/NUP214
Deletion 13q
Deletion 17p
Deletion 20q
ELL/MLL
ENL/MLL
idic(20)
isodicentric 20q
MLLT1/MLL
MLLT4/MLL
MLLT3/MLL
MLLT10/MLL
MLL/CREBBP
Monosomy 5
Monosomy 7
PRDM16/RPN1
RBM15/MKL1
t(1;3)
t(1;22)
t(6;11)
t(10;11)
t(11;16)
t(11;19)
TP53
Trisomy 8
MLF1/NPM1
t(3;5)