Acute Myeloid Leukemia (AML), FISH
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Detecting a neoplastic clone associated with the common chromosome anomalies seen in patients with acute myeloid leukemia or other myeloid malignancies
Evaluating specimens in which standard cytogenetic analysis is unsuccessful
Identifying and tracking known chromosome anomalies in patients with myeloid malignancies and tracking response to therapy
Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)
|Test ID||Reporting Name||Available Separately||Always Performed|
|ADD1F||One Additional FISH Probe||No||No|
|ADD2F||Two Additional FISH Probes||No||No|
|ADD4F||Four Additional FISH Probes||No||No|
|ADD3F||Three Additional FISH Probes||No||No|
|ADD5F||Five Additional FISH Probes||No||No|
|ADD6F||Six Additional FISH Probes||No||No|
|ADD7F||Seven Additional FISH Probes||No||No|
|ADD8F||Eight Additional FISH Probes||No||No|
|ADD9F||Nine Additional FISH Probes||No||No|
|ADD10||Ten Additional FISH Probes||No||No|
|ADD11||Eleven Additional FISH Probes||No||No|
|ADD12||Twelve Additional FISH Probes||No||No|
|14FP||Fourteen Additional FISH Probes||No||No|
|13FP||Thirteen Additional FISH Probes||No||No|
|15FP||Fifteen Additional FISH Probes||No||No|
|16FP||Sixteen Additional FISH Probes||No||No|
|17FP||Seventeen Additional FISH Probes||No||No|
|18FP||Eighteen Additional FISH Probes||No||No|
|19FP||Nineteen Additional FISH Probes||No||No|
|20FP||Twenty Additional FISH Probes||No||No|
|21FP||Twenty One Additional FISH Probes||No||No|
|22FP||Twenty Two Additional FISH Probes||No||No|
|23FP||Twenty Three Additional FISH Probes||No||No|
|24FP||Twenty Four Additional FISH Probes||No||No|
|25FP||Twenty Five Additional FISH Probes||No||No|
|26FP||Twenty Six Additional FISH Probes||No||No|
|27FP||Twenty Seven Additional FISH Probes||No||No|
|28FP||Twenty Eight Additional FISH Probes||No||No|
|29FP||Twenty Nine Additional FISH Probes||No||No|
|30FP||Thirty Additional FISH Probes||No||No|
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
See Acute Promyelocytic Leukemia: Guideline to Diagnosis and Follow-up in Special Instructions.
When this test is ordered, a charge for 2 FISH probes and interpretation is included. If additional probes or the entire panel are ordered, additional probe charges will be added.
Please indicate if the entire panel is to be performed. This is suggested for patients with a new diagnosis of acute myeloid leukemia. If the patient is being treated for known anomalies, indicate which anomalies need to be investigated.
Indicate subtype as well as which anomalies need to be investigated from the following profile:
t(8:21), [M2], RUNX1T1/RUNX1
t(15:17), [M3], PML/RARA
t(11q23:var), [M0-M7], MLL
inv(16), [M4, Eos], MYH11/CBFB
+8, [M0-M7], Cen8/MYC
t(6;9), [M2, M4], DEK/NUP214
inv(3), [M1, M2, M4, M6, M7], RPN1/MECOM
t(8;16), [M4, M5], MYST3/CREBBP
t(3;5), [M2, M4, M6], MLF1/NPM1
t(1;22), [M7], RBM15/MKL1*
*The RBM15/MKL1 probe set will only be used to test patients with a suspected or confirmed diagnosis of M7 or to confirm a t(1;22) identified by chromosome analysis.
-When an MLL rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(6;11)(q27;q23) MLLT4/MLL, t(9;11)(p22;q23) MLLT3/MLL, t(10;11)(p13;q23) MLLT10/MLL, t(11;16)(q23;p13.3) MLL/CREBBP, t(11;19)(q23;p13.1) MLL/ELL, or t(11;19)(q23;p13.3) MLL/MLLT1.
-When 3 copies of MECOM are observed with no fusion with RPN1, reflex testing using the MECOM/RUNX1 probe set is performed to identify a potential t(3;21)(q26.2;q22) rearrangement.
-When 3 copies of RPN1 are observed with no fusion with MECOM, reflex testing using the PRDM16/RPN1 probe set is performed to identify a potential t(1;3)(p36;q21).
This assay detects chromosome abnormalities observed in the blood and bone marrow of patients with acute myeloid leukemia. For testing paraffin-embedded tissue samples from patients with myeloid sarcoma, see FMST / Myeloid Sarcoma, FISH, Tissue.
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Fluorescence In Situ Hybridization (FISH)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Acute Myeloid Leukemia (AML)
Acute Promyelocytic Leukemia (APL)
APL (Acute Promyelocytic Leukemia)
FISH (Fluorescence In Situ Hybridization)