Test ID: IDENT
Organism Referred for Identification, Aerobic Bacteria
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Identification of pure isolates of aerobic bacteria
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Method Name A short description of the method used to perform the test
Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, and 16S RNA sequencing.
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen Type: Pure culture of organism from source cultured
Container/Tube: Sterile broth/culture media
Specimen Volume: Entire specimen
Collection Instructions:
1. Isolate infecting bacteria.
2. Organism must be in pure culture, actively growing.
3. Place specimen in a large infectious container (Supply T146) and label as an etiologic agent.
Additional Information:
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
3. If susceptibility testing is needed; order ZMMLS Antimicrobial Susceptibility, Aerobic Bacteria, MIC also. If susceptibilities are not appropriate, ZMMLS will be canceled at report time.
4. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Agar plate |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Organisms are referred to confirm an identification or when an identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.
Techniques employed may include conventional biochemical analysis, fluorescent-antibody staining, carbon source utilization, and sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Identification of organism
Interpretation Provides information to assist in interpretation of the test results
Genus and species are reported on aerobic bacterial isolates, whenever possible.
Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless speciation is noted or specifically requested on the request form.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
No significant cautionary statements
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Manual of Clinical Microbiology. Edited by: PR Murray, EJ Baron, MA Pfaller, et al: Manual of Clinical Microbiology. Ninth edition. Washington, DC, ASM Press, 2007
2. Koneman EW, Allen SD, Janda WM, et al: In Color Atlas and Textbook of Diagnostic Microbiology. Fifth edition. New York, Lippincott-Raven Publishers, 1997
Method Description Describes how the test is performed and provides a method-specific reference
Pure isolates of aerobic organisms received on slants are inoculated onto culture plates based on Gram-stain morphology, the source of the isolate, clinical history, and previous results submitted by the referring client. In general, routine media utilized includes chocolate blood agar, sheep blood agar, and eosin methylene blue agar. After incubation at 35 degrees C in 5% CO2, the organism is identified using 1 or a combination of the following techniques: conventional biochemical tests, fluorescent-antibody staining, carbon source utilization, and sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.(Manual of Clinical Microbiology. Seventh edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al: Washington, DC, ASM Press, 1999; RS Weyant, CW Moss, RE Weaver, et al: Identification of unusual pathogenic gram negative aerobic and facultatively anaerobic bacteria. CDC, Atlanta, GA, 1996; Bergey's Manual of Systematic Bacteriology, Vol 1. Edited by NR Krieg. Baltimore, MD, Williams and Wilkins, 1984; Kolbert CP, Persing DH: Ribosomal DNA sequencing as a tool for identification of bacterial pathogens. Curr Opin Microbiol 1999;2:299-305)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
Organism Referred for Identification, Aerobic Bacteria
87077
Gram negative bacillus identification
87077 (if appropriate)
Identification commercial kit
87077 (if appropriate)
Identification by MALDI-TOF mass spectrometry
87077 (if appropriate)
Fluorescent method culture typing
87140 (if appropriate)
Fluorescent method culture typing
87140 (if appropriate)
Bacteria identification
87077 (if appropriate)
Aerobe identification by sequencing
87153 (if appropriate)
Additional identification procedure
87077 (if appropriate)
Serologic agglutination method identification
87147 (if appropriate)
Serologic agglutination method identification
87147 (if appropriate)
Identification staphylococcus
87077 (if appropriate)
Identification streptococcus
87077 (if appropriate)
Beta lactamase
87185 (if appropriate)
KPC PCR
87150 (if appropriate)
Hodge Test
87185 (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| IDENT | Organism Refer for ID, Aerobic Bact | In Process |
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