Test ID: FFES
Estradiol Free, Serum (includes Estradiol and SHBG)
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Method Name A short description of the method used to perform the test
Mass Spectrometry
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Free Estradiol, Serum FORWARD
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Draw blood in a plain, red-top tube or serum gel tube. Spin down, pour off into plastic vial within 1 hour of collection, and send 3 mL serum frozen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Specimens other than | Serum |
| Anticoagulants other than | NA |
| Hemolysis | NA |
| Thawing | NA |
| Lipemia | NA |
| Icteric | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 180 days |
| Ambient | 5 days | |
| Refrigerated | 5 days |
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Free Estradiol, Percent
Reference Ranges (%)
Age Range
Adult Males 1.7 - 5.4
Adult Females 1.6 - 3.6
Free Estradiol, Serum
Reference Ranges (pg/mL)
Age Range
Adult Males 0.2 - 1.5
Adult Females 0.6 - 7.1
Sex Hormone Binding Globulin (SHBG), Serum
Reference Ranges (nmol/L)
Age Range
1 - 23m 60 - 252
Prepubertal (24m - 8y) 72 - 220
Pubertal
Males 16 - 100
Females 36 - 125
Adult Males 20 - 60
Adult Females
Premenopausal 40 - 120
Postmenopausal 28 - 112
Estradiol, Serum
Reference Ranges (pg/mL)
Age Range
Newborn Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15
Males <6m Levels increase to 10 - 32 between 30 and 60 days, hen decline to prepubertal levels of <15 by six months.
Females <1y Levels increase to 5.0 - 50 between 30 and 60 days, then decline to prepubertal levels of <15 during the first year.
Prepubertal <15
Adult Males 8.0 - 35
Adult Females
Follicular 30 - 100
Luteal 70 - 300
Postmenopausal <15
Test Performed By: Esoterix Endocrinology
4301 Lost Hills Road
Calabasas Hills, CA 91301
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
82670/Estradiol
84270/Sex Hormone binding globulin
84999/Unlisted chemistry procedure
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| Z0228 | Free Estradiol, Percent | 14960-9 |
| Z0229 | Free Estradiol, Serum | 2240-0 |
| Z0230 | Sex Hormone Binding Globulin (SHBG) | 13967-5 |
| Z0259 | Estradiol, Serum | 35384-7 |
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