Test ID: PLHBB
Plasma Hemoglobin, Plasma
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Determining whether hemolysis is occurring such as from:
-Transfusion reaction
-Mechanical fragmentation of red blood cells
Method Name A short description of the method used to perform the test
Spectrophotometry (SP)
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Hemoglobin, Plasma
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 2 mL
Collection Instructions: Spin down within 2 hours of draw.
Forms: If not ordering electronically, please submit a Hematopathology/Molecular Oncology Request Form (Supply T241) with the specimen.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | Mild OK; Gross OK |
| Lipemia | Mild OK; Gross OK |
| Icterus | NA |
| Other | Serum |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days | |
| Ambient | 48 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Plasma normally contains no free hemoglobin ie, no hemoglobin that is not contained in erythrocytes.
Significant amounts of hemoglobin occur in plasma following hemolysis such as might result from a transfusion reaction or mechanical fragmentation of red blood cells during cardiac surgery.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
TOTAL HEMOGLOBIN
> or =12 months: 0.0-15.2 mg/dL
Reference values have not been established for patients who are less than 12 months of age.
OXYHEMOGLOBIN
> or =12 months: 0.0-12.4 mg/dL
Reference values have not been established for patients who are less than 12 months of age.
Interpretation Provides information to assist in interpretation of the test results
Total hemoglobin:
> or =12 months: 0.0-15.2 mg/dL
Reference values have not been established for patients who are less than 12 months of age.
Oxyhemoglobin:
> or =12 months: 0.0-12.4 mg/dL
Reference values have not been established for patients who are less than 12 months of age.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Test should not be performed on serum; hemoglobin is liberated from red blood cells during clotting.
Drawing blood specimens for plasma hemoglobin measurement should be done with considerable care to avoid causing hemolysis.
Bilirubin is a known interference with this assay. Elevated bilirubin levels interfere with the quantitation of the total plasma hemoglobin.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Fairbanks VF, Ziesmer SC, O'Brien PC: Methods for measuring plasma hemoglobin in micromolar concentration compared. Clin Chem 1992;38:132-140
Method Description Describes how the test is performed and provides a method-specific reference
Plasma is examined spectrophotometrically at 8 wavelengths ranging from 415 nm to 700 nm, and calculations are made that permit quantitation of total heme pigments, oxyhemoglobin, total bilirubin, and methemalbumin, comprising all the frequently encountered plasma pigments.(Fairbanks VF, Ziesmer S, O'Brien PC: Methods for measuring plasma hemoglobin in micromolar concentration compared. Clin Chem 1992;38:132-140)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
83051
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| 31970 | Total Hemoglobin | 721-1 |
| 31971 | Oxyhemoglobin | In Process |
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