Test ID: ABTIH
Antibody Titer, Erythrocytes
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Monitoring antibody levels during pregnancy to help assess the risk of hemolytic disease of the newborn
Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ABID2 | Antibody Identification, RBC | Yes | No |
| DCTM | Direct Coombs, B | Yes | No |
| ABAB | Antibody Absorption | No | No |
| ABEL | Antibody Elution | No | No |
| MDCC3 | Monospecific Direct Coombs C3, B | Yes | No |
| MDCG | Monospecific Direct Coombs IgG, B | Yes | No |
| SPAG | Special Red Cell Ag Typing | Yes | No |
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
The initial test performed is an antibody identification. If the antibody problem identified is not relevant in hemolytic disease of the newborn, or if titrations are not helpful, the titer will be cancelled and will be replaced by the antibody identification test. Depending upon the antibody identification results, additional tests, ie, monospecific direct Coombs, antibody elution, antibody absorption, and red cell antigen typing may be added and charged. A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Method Name A short description of the method used to perform the test
ABTIH/9000: Titration
ABID2/8988: Ten-Cell Panel
ABA/9389, SPAG/8980: Standard Blood Bank Techniques
ABEL/9390: Acid-EDTA Elution/Other standard methods may be used in difficult cases.
DCTM/9008, MDCG/86880, MDCC3/87855: Direct Antiglobulin Test
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Lewis A Antibody Titer
RBC Antibody Titer
Rh (Rhesus) Titer
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Include report of diagnosis and history of transfusions and pregnancy. If some antibody panel work has been done, include copies of the work.
Specimen must arrive within 72 hours of draw.
Blood and serum are required.
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 7 mL
Collection Instructions: Spin down and separate plasma from cells. Send both tubes.
Specimen Type: Serum
Container/Tube: Red top
Specimen Volume: 10 mL
Collection Instructions: Spin down and separate serum from clot. Send both tubes.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Serum gel tube |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | 4 days |
| Refrigerated | 4 days |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Some maternal IgG alloantibodies to red blood cell antigens will cross the placenta and cause hemolysis of antigen-positive fetal red cells. The resulting fetal anemia and hyperbilirubinemia can be harmful or even fatal to the fetus or the newborn.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
If positive, result will be reported as the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.
Interpretation Provides information to assist in interpretation of the test results
The specificity of the maternal alloantibody will be stated. The titer result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed.
If the antibody problem identified is not relevant in hemolytic disease of the newborn or if titrations are not helpful, the titer will be canceled and will be replaced by ABID2/8988 Antibody Identification, Erythrocytes.
A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Not useful for evaluating the efficacy of Rh-immune globulin after it has been administered
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Technical Manual. Bethesda, MD, American Association of Blood Banks
Method Description Describes how the test is performed and provides a method-specific reference
The specificity of the antibody to be titered is first determined and optimal phases of reactivity are selected. Two-fold serial dilutions of serum are tested against antigen-positive erythrocytes under optimal conditions. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed. Parallel titration of a previous specimen of the patient's serum (frozen) provides a baseline for comparison of antibody level. In the absence of a previous specimen from the patient, parallel titration of a control antiserum is used for standardization.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; Continuously, Saturday; 12 a.m.-4 p.m.,
Sunday; 7 a.m.-12 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
86870-Antibody identification
86860-Antibody elution (if appropriate)
86880 x 3-Antiglobulin, direct (if appropriate)
86886-Antibody titer (if appropriate)
86905-Each red cell antigen typing (if appropriate)
86978-Absorption, each (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| ABTI | Antibody Titer, RBC | In Process |
| TITE | Titer | 50401-9 |
| TITEP | Titer of Previous Sample | 50401-9 |
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