Antibody Identification, Erythrocytes
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Assessing positive pretransfusion antibody screens, transfusion reactions, hemolytic disease of the newborn, and autoimmune hemolytic anemias
Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)
|Test ID||Reporting Name||Available Separately||Always Performed|
|DCTM||Direct Coombs, B||Yes||No|
|MDCC3||Monospecific Direct Coombs C3, B||Yes||No|
|MDCG||Monospecific Direct Coombs IgG, B||Yes||No|
|SPAG||Special Red Cell Ag Typing||Yes||No|
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
The following tests may also be ordered and performed as part of antibody identification: special red cell antigen typing, antibody elution, antibody absorption, direct Coombs, and monospecific direct Coombs. These additional tests will determine or confirm antibody specificity and allo- vs. autoantibody.
ABID2/8988: Ten-Cell Panel
ABAB/9389, SPAG/8980: Standard Blood Bank Techniques
ABEL/9390: Acid-EDTA Elution/Other standard methods may be used in difficult cases.
DCTM/9008, MDCG/86880, MDCC3/87855: Direct Antiglobulin Test
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Antibody Identification, RBC
Red Cell Antibody Identification
Red Cell Antibody Identification
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Include report of diagnosis and history of transfusions and pregnancy. If some antibody panel work has been done, include copies of the work.
Specimen must arrive within 72 hours of draw.
Blood and serum are required.
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 18 mL
Specimen Minimum Volume: 6 mL
Collection Instructions: Send specimen in original tube.
Specimen Type: Serum
Container/Tube: Red top
Specimen Volume: 10 mL
Collection Instructions: Spin down and separate serum from clot.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Blood: 6 mL
Serum gel tube
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Varies||Ambient (preferred)||4 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
After exposure to foreign red blood cells via transfusion or pregnancy, some people form antibodies which are capable of the destruction of transfused red cells or of fetal red cells in utero. It is important to identify the antibody specificity in order to assess the antibody's capability of causing clinical harm and, if necessary, to avoid the antigen on transfused red blood cells.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
If positive, antibodies will be identified and corresponding special red cell antigen typing on patient's red blood cells will be performed. A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Specificity of alloantibodies will be stated.
The patient's red blood cells will be typed for absence of the corresponding antigen(s) or as an aid to identification in complex cases.
A consultation service is offered, at no charge, regarding the clinical relevance of red cell antibodies.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Recent administration of Rh-immune globulin may cause anti-D to be identified and appear falsely as an alloantibody
Not useful for monitoring the efficacy of Rh-immune globulin administration
Not useful for identifying antibodies detected only at 4 degrees C or only after extended room temperature incubation
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Technical Manual. Bethesda, MD, American Association of Blood Banks
Method Description Describes how the test is performed and provides a method-specific reference
A panel of reagent type O erythrocytes, with known antigenic determinants and the patient's cells are tested with the patient's serum/plasma. This panel should yield a distinct pattern of agglutination or hemolysis that identifies the auto- or alloantibody specificity. Elution, absorption, neutralization, and other special techniques may be necessary to complete antibody identification.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; Continuously, Saturday; 12 a.m.-4 p.m.,
Sunday; 7 a.m.-12 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
86860-Antibody elution (if appropriate)
86880 x 3-Antiglobulin, direct (if appropriate)
86905-Each red cell antigen typing (if appropriate)
86978-Absorption (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|ABIDW||Antibody Identification, RBC||888-8|