Test ID: VIBC
Vibrio Culture, Stool
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Determining whether Vibrio species is the cause of diarrhea and, in turn, identifying the source of the infectious agent
Method Name A short description of the method used to perform the test
Conventional Culture Technique
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Vibrio cholerae
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Specimen must arrive within 72 hours of collection.
Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, such as Cary-Blair).
Specimen Volume: Representative portion of stool specimen
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Stool swab, stool in Ecofix (green cap), Preserved stool >72 hours, specimen in buffered glycerol saline transport media |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Fecal | Ambient | 72 hours |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Diarrhea may be caused by a number of agents (eg, bacteria, viruses, parasites, and chemicals) and these agents may result in similar symptoms. A thorough patient history covering symptoms, severity and duration of illness, age, travel history, food consumption, history of recent antibiotic use, and illnesses in the family or other contacts will help the physician determine the appropriate testing to be performed.
Vibrio cholerae, the causative agent of endemic, epidemic, and pandemic cholera, results in large volumes of rice-water stools due to the production of an enterotoxin. Severe dehydration is of concern in patients without access to adequate medical care. In the United States, Vibrio parahemolyticus is the most common cause of Vibrio disease. Vibrio parahemolyticus is associated with the consumption of raw shellfish or fish and results in gastroenteritis with nausea, vomiting, abdominal cramps, low-grade fever, and chills. Usually rehydration is the only treatment required, although in some cases, antimicrobial therapy is needed.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
No growth
Interpretation Provides information to assist in interpretation of the test results
The growth of Vibrio species identifies the cause of diarrhea.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
The yield of enteric pathogens is reduced when specimens are delayed in transit to the laboratory (>2 hours from collection for unpreserved specimen).
Buffered glycerol saline is an unacceptable transport media for Vibrio culture.
Supportive Data
(Clinical pathologic correlative studies.)
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Mandell GL, Bennett JE, Dolin R: In Principle's and Practice of Infectious Disease. Sixth edition. Philadelphia, Elsevier Inc. 2005, pp 2536-2548
Method Description Describes how the test is performed and provides a method-specific reference
The fecal specimen is inoculated onto selective media, thiosulfate-citrate-bile salts-sucrose agar (TCBS), designed to inhibit growth of normal bowel flora while allowing growth of Vibrio species. Suspect colonies are identified using conventional biochemical methods.(Murray PR, Baron EJ, Jorgensen JH, et al: In Manual of Clinical Microbiology. Nineth edition. Washington, DC, ASM Press, 2007, pp 723-733)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
87081-Vibrio culture, stool
87077-Identification by MALDI-TOF mass spec (if appropriate)
87077-Additional identification procedure (if appropriate)
87153-Aerobe identification by sequencing (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| VIBC | Vibrio Culture, Stool | In Process |
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