Test ID: PF
Chromosomes, Body Fluid
Secondary ID 
A test code used for billing and in test definitions created prior to November 2011
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Assisting in the diagnosis of certain malignancies
Method Name A short description of the method used to perform the test
Includes 2 banded karyograms, analysis of 20 or more
metaphases, and other techniques when required.
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Chromosome Analysis, body fluid
Chromosome Analysis, pleural fluid
Chromosome Analysis, spinal fluid
Karyotype, body fluid
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Provide a reason for referral and the specimen source with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Container/Tube: Sterile container
Specimen Volume: 25-50 mL
Collection Instructions: Collect specimen in a sterile syringe.
Additional Information: Advise Express Mail or equivalent, if not on courier service.
Forms: If not ordering electronically, please submit the following forms with the specimen:
-Cytogenetics/AFP Congenital Disorders Request Form (Supply T238) with the specimen
-Cytogenetics Hematologic Disorders Request Form (Supply T607)
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | NA |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Body Fluid | Refrigerated (preferred) | |
| Ambient | ||
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Cytogenetic studies on body fluids (eg, pleural effusions, ascites, and pericardial, cerebrospinal, and synovial fluids) may be helpful to diagnose or to rule-out metastases or relapses in patients with lymphoma or other malignancies.
Chromosome analysis serves as a useful adjunct to cytology. In pleural fluids, lymphomas are often more readily diagnosed by cytogenetic techniques than by standard cytologic examination.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
46,XX or 46,XY. No apparent chromosome abnormality. An interpretive report will be provided.
Interpretation Provides information to assist in interpretation of the test results
The observation of a chromosomally abnormal clone is evidence of a clonal neoplastic process.
A normal karyotype does not eliminate the possibility of a neoplastic process.
On rare occasions, the presence of an abnormality may be associated with a congenital abnormality that is not related to a malignant neoplastic process. Follow-up with a medical genetics consultation is recommended.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Interfering factors
Technical:
-Lack of viable cells
-Bacterial contamination
-Cell death due to failure to transport tissue in appropriate media
-Excessive transport time
-Exposure of the specimen to temperature extremes (freezing or >30 degrees C).
Biological:
-Normal cells may grow better in culture than tumor cells and interfere with the cytogenetic studies.
-Subtle structural chromosome anomalies can be missed occasionally
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Dewald GW, Dines DE, Weiland LH, Gordon H: The usefulness of chromosome examination in the diagnosis of malignant pleural effusions. N Engl J Med 1976;295:1494-1500
Method Description Describes how the test is performed and provides a method-specific reference
The tissue is centrifuged and the cell pellet is placed in culture medium. After 24 to 48 hours, the cultures may be harvested or a longer-term fibroblast culture may be established and harvested within 3 to 20 days or longer. The harvest procedure involves treating the cells with colcemid and hypotonic solution, and then fixing the cells with glacial acetic acid and methanol. Metaphase cells are dropped onto microscope slides and are routinely stained by G- or Q-banding. Twenty metaphases are usually examined. If a clone is suspected but not confirmed in 20 metaphases, an additional 10 cells will be analyzed. Minimal evidence for the presence of an abnormal clone is defined as 2 or more metaphases with the same structural abnormality or chromosome gain (trisomy), or 3 or more metaphases lacking the same chromosome. Five to 10 metaphases are captured using a computerized imaging system, and 1 or more karyograms from each clone are prepared to document the type of abnormality and to permit systematic interpretation of the anomalies. (Dewald GW, Hicks GA, Dines D, Gordon H: Usefulness of culture methods to aid cytogenetic analyses for the diagnosing of malignant pleural effusions. Mayo Clin Proc 1982;57:488-494)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
88239-Tissue culture for tumor
88291-Interpretation and report
88299-Unlisted cytogenetic study (Refer to patient report to apply the appropriate CPT code below in place of this unlisted cytogenetic study CPT code)
Based on the total number of cells analyzed and counted, MML would recommend the following:
Chromosome analysis with less than 5 cells; CPT Code 88261 w/ modifier 52
Chromosome analysis with 5 to 14 cells; CPT Codes 88261, 88285
Chromosome analysis with 15 to 19 cells; CPT Code 88262
Chromosome analysis with 20 to 25 cells; CPT Code 88264
Chromosome analysis with more than 25 cells; CPT Codes 88264, 88285
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| 16119 | Specimen | 31208-2 |
| 16333 | Specimen ID | N/A |
| 16788 | Source | N/A |
| 16120 | Order Date | N/A |
| CG345 | Reason For Referral | 42349-1 |
| 16124 | Method | 49549-9 |
| 50470 | Banding Methods | 62359-5 |
| 16126 | Results | 35129-6 |
| 16127 | Interpretation | 69965-2 |
| 16128 | Amendment | In Process |
| 16129 | Consultation | In Process |
| 16130 | Report Date | N/A |
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