Ribosome P Antibodies, IgG, Serum
NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.
As an adjunct in the evaluation of patients with lupus erythematosus (LE)
As an aid in the differential diagnosis of neuropsychiatric symptoms in patients with LE
Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.
See Connective Tissue Disease Cascade (CTDC) in Special Instructions.
Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test
Multiplex Flow Immunoassay
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Ribosome P Ab, IgG, S
Anti-Ribosome P Antibodies
Ribosomal P Antibodies
Ribosomal P Antibodies
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 0.5 mL
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Mild OK; Gross reject
Mild OK; Gross reject
Mild OK; Gross OK
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
|Serum||Refrigerated (preferred)||21 days|
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
The 80S mammalian ribosome is composed of approximately equal amounts of protein and RNA. The larger 60S subunit contains 3 acidic phosphoproteins, PO, P1, and P2 with molecular masses of 38 kDa, 19 kDa, and 17 kDa, respectively. The major immunoreactive epitope of these 3 autoantigens is found within 22 consecutive amino acids of the carboxy terminus of these 3 highly conserved proteins.
It has been known for some time that sera from some patients with lupus erythematosus (LE) react with ribosomal protein antigens. Studies performed with synthetic peptide antigens revealed that reactivity detected by immunoprecipitation and by immunofluorescence methods in sera from LE patients was directed at the above mentioned epitope. Antibodies to ribosome P proteins are considered highly specific for LE, and have been reported in patients with central nervous system (CNS) involvement and so called "lupus psychosis." The reported frequency of antibodies to ribosome P protein autoantigens in patients with LE is approximately 12%.
Since patients with LE may manifest signs and symptoms of CNS diseases including neuropsychiatric symptoms, the presence of antibodies to ribosome P protein may be useful in the differential diagnosis of such patients. Other causes of CNS symptoms in patients with LE include thrombosis with or without antibodies to phospholipid antigens and iatrogenic effects from treatment with corticosteroid drugs.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
<1.0 U (negative)
> or =1.0 U (positive)
Reference values apply to all ages.
A positive result is consistent with the diagnosis of lupus erythematosus, and may indicate the presence of central nervous system involvement.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Most patients with lupus erythematosus (LE) do not have detectable levels of antibodies to ribosome P protein.
This test should not be relied upon to establish the diagnosis or to rule out the diagnosis in a patient with signs and symptoms compatible with LE.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
1. Bonfa E, Golombek SJ, Kaufman LD, et al: Association between lupus psychosis and anti-ribosomal P protein antibodies. N Engl J Med 1987;317:265-271
2. Bonfa E, Elkon KB: Clinical and serologic associations of the anti-ribosomal P protein antibody. Arthritis Rheum 1986;29:981-985
Method Description Describes how the test is performed and provides a method-specific reference
Affinity-purified ribosome P antigens are coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. Ribosome P antibodies, if present in diluted serum, bind to ribosome P antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated, antihuman IgG antibody is then added to detect IgG anti-ribosome P antibodies bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for ribosome P microspheres to a 4-point calibration curve. (Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories, Hercules, CA)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 4 p.m., Sunday; 11a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
|Result ID||Reporting Name||LOINC Code|
|RIB||Ribosome P Ab, IgG, S||53892-6|