Test ID: FDERM
Fungal Culture, Dermal
NY State Approved 
Indicates the status of NY State approval and if the test is orderable for NY State clients.
Useful For Suggests clinical disorders or settings where the test may be helpful
Recovery and identification of dermatophyte fungi from hair, skin, and nail infection specimens
Method Name A short description of the method used to perform the test
Plated to Mycobiotic Agar
Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name
Aliases Lists additional common names for a test, as an aid in searching
Culture, Yeast
Dermatophytes
Fungus Culture
Mold
Mycology, Culture
Yeast Culture
50021-FDERM
Specimen Type Describes the specimen type needed for testing
Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.
Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.
Specimen must arrive within 7 days of collection.
Specimen source is required.
Specimen Type: Hair
Container/Tube: Sterile container
Specimen Volume: 10-12
Collection Instructions: Using forceps collect affected hairs with shaft intact.
Specimen Type: Nails
Container/Tube: Sterile container
Specimen Volume: Entire collection
Collection Instructions:
1. Wipe the nail with 70% alcohol using gauze (not cotton).
2. Clip away a generous portion of the affected area.
3. Collect material or debris from under the nail.
Specimen Type: Skin
Container/Tube: Sterile container
Specimen Volume: Entire specimen
Collection Instructions:
1. Cleanse the affected area with 70% alcohol.
2. Gently scrape the surface of the skin at the active margin of the lesion, being careful to not draw blood.
Specimen Minimum Volume Defines the amount of specimen required to perform an assay once, including instrument and container dead space. Submitting the minimum specimen volume makes it impossible to repeat the test or perform confirmatory or perform reflex testing. In some situations, a minimum specimen volume may result in a QNS (quantity not sufficient) result, requiring a second specimen to be collected.
Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected
| Hemolysis | NA |
| Lipemia | NA |
| Icterus | NA |
| Other | Charcoal, wooden shaft, or dry swab |
Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient | 7 days |
Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test
Fungal infections of keratinized tissues (hair, skin, nails) can be caused by dermatophytic fungi belonging to the genera Epidermophyton, Microsporum, and Trichophyton. Opportunistic superficial infections resembling dermatophytoses may be caused by yeasts or by unrelated filamentous fungi that are normally saprobes or plant pathogens. Dermatophytes are usually unable to penetrate deeper tissues. Infection may range from mild to severe.
Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.
Negative
If positive, fungus or yeast will be identified.
Interpretation Provides information to assist in interpretation of the test results
Positive cultures are reported with organism identification.
Negative reports are issued after 30 days incubation.
Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances
Aseptic techniques should be used when collecting specimens to minimize contamination. For optimal recovery of organisms, sufficient clinical material should be collected.
Clinical Reference Provides recommendations for further in-depth reading of a clinical nature
Summerbell RC, Weitzman I, Padhye AA: Trichophyton, Microsporum, Epidermophyton and agents of superficial mycoses. In Manual of Clinical Microbiology. Ninth edition. Edited by PR Murray, EJ Baron, et al: Washington DC. ASM Press, 2007, pp 1874-1897
Method Description Describes how the test is performed and provides a method-specific reference
Specimens are plated on mycobiotic agar, which contains chloramphenicol and cyclohexamide to inhibit bacterial and saprobic fungal contamination. Cultures are incubated at 30 degrees C for 30 days. Identification of dermatophyte species is based on colony and microscopic morphology and a variety of available physiologic tests.
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Performing Laboratory Location The location of the laboratory that performs the test
Test Classification Provides information regarding the medical device classification for laboratory test kits and reagents. Tests may be classified as cleared or approved by the US Food and Drug Administration (FDA) and used per manufacturer's instructions, or as products that do not undergo full FDA review and approval, and are then labeled as an Analyte Specific Reagent (ASR), Investigation Use Only (IUO) product, or a Research Use Only (RUO) product.
CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.
87101-Fungal culture, dermal
87106-Yeast identification panel C (if appropriate)
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87107-Yeast identification panel A (if appropriate)
87107-Yeast identification panel B (if appropriate)
87150-Identification rapid PCR coccidioides (if appropriate)
87150 x 2- Identification Histoplasma/Blastomyces, PCR (if appropriate)
87150-Microbial probe, fungus identification (if appropriate)
87153- D2 fungal sequencing identification (if appropriate)
LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the result codes returned for this test or profile.
| Result ID | Reporting Name | LOINC Code |
|---|---|---|
| FDERM | Fungal Culture, Dermal | In Process |
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